A total of 43 urine samples were examined for the presence of uropathogenic bacteria. Escherichia coli was isolated from 33 of these samples, while Klebsiella species were found in seven (7). Bacterial identification was confirmed using standard biochemical tests following CLSI guidelines. E. coli tested positive for Indole and Catalase, whereas Klebsiella spp. were negative for Indole and Oxidase; both organisms showed lactose fermentation on MacConkey agar. All confirmed isolates were screened for Metallo-?-lactamase (MBL) and Extended-Spectrum ?-lactamase (ESBL) production. MBL activity was observed in two E. coli isolates using the Meropenem-EDTA Disk Synergy Test, indicated by a ?7 mm increase in the inhibition zone. ESBL production was confirmed in one E. coli and three Klebsiella isolates using the Double Disc Diffusion Test with Ceftriaxone and Clavulanic Acid, where a zone enhancement of ?8 mm signified ESBL activity. Three selected isolates underwent Sanger sequencing and were analyzed through BLAST. These results underscore the presence of multidrug-resistant pathogens in urinary tract infections and highlight the importance of ongoing antimicrobial resistance surveillance.
